Spectrophotometer
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Explain the principle of spectrophotometer and describe its following components: (i) Radiation sources (ii) Phototube (iii) Prism (UPSC 2020, 15 Marks, )
स्पेक्ट्रोफोटोमीटर के सिद्धांत को समझाएं और इसके निम्नलिखित घटकों का वर्णन करें: (i) विकिरण स्रोत (ii) फोटो ट्यूब (iii) प्रिज्म। (Explain the principle of spectrophotometer and describe its following components: (i) Radiation sources (ii) Phototube (iii) Prism.)Answer
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What is the principle of spectrophotometry? Discuss the details of instrumentation and application of spectrophotometer. Comment upon the common feature of spectrophotometric assay and ELISA, if any. (150 words) (UPSC 2013, 25 Marks, )
स्पेक्ट्रोफोटोमेट्री का सिद्धांत क्या है? स्पेक्ट्रोफोटोमीटर के उपकरण और अनुप्रयोग के विवरण पर चर्चा करें। स्पेक्ट्रोफोटोमेट्रिक एस्से और ELISA की सामान्य विशेषता पर टिप्पणी करें, यदि कोई हो। (150 शब्द)। (English Meaning in bracket)Answer
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Discuss the theoretical basis of spectrophotometry with reference to the Beer-Lambert law. Write an account of the Instrumentation, maintenance, and application of spectrophotometer. (UPSC 2011, 30 Marks, )
स्पेक्ट्रोफोटोमेट्री के सैद्धांतिक आधार पर चर्चा करें, विशेष रूप से बीयर-लैम्बर्ट नियम के संदर्भ में। स्पेक्ट्रोफोटोमीटर के उपकरण, रखरखाव, और अनुप्रयोग का विवरण लिखें। (Discuss the theoretical basis of spectrophotometry with reference to the Beer-Lambert law. Write an account of the Instrumentation, maintenance, and application of spectrophotometer.)Enroll Now
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What is Lambert-Beer's Law? Discuss the construction of spectrophotometer. Add a note on the applications of UV-visible spectrophotometry in biological studies. (UPSC 2005, 30 Marks, )
लैम्बर्ट-बियर का नियम क्या है? स्पेक्ट्रोफोटोमीटर की संरचना पर चर्चा करें। जैविक अध्ययनों में यूवी-दृश्य स्पेक्ट्रोफोटोमेट्री के अनुप्रयोगों पर एक नोट जोड़ें। (What is Lambert-Beer's Law? Discuss the construction of spectrophotometer. Add a note on the applications of UV-visible spectrophotometry in biological studies.)Enroll Now